Using virtual filtering approach to discriminate microalgae by spectral flow cytometer

Natasha Barteneva, Aigul Kussanova, Veronika Dashkova, Ayagoz Meirkhanova, Ivan Vorobjev

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)peer-review

2 Citations (Scopus)

Abstract

Fluorescence methods are widely used for the study of marine and freshwater phytoplankton communities. However, the identification of different microalgae populations by the analysis of autofluorescence signals remains a challenge. Addressing the issue, we developed a novel approach using the flexibility of spectral flow cytometry analysis (SFC) and generating a matrix of virtual filters (VF) which allowed thorough examination of autofluorescence spectra. Using this matrix, different spectral emission regions of algae species were analyzed, and five major algal taxa were discriminated. These results were further applied for tracing particular microalgae taxa in the complex mixtures of laboratory and environmental algal populations. An integrated analysis of single algal events combined with unique spectral emission fingerprints and light scattering parameters of microalgae can be used to differentiate major microalgal taxa. We propose a protocol for the quantitative assessment of heterogenous phytoplankton communities at the single-cell level and monitoring of phytoplankton bloom detection using a virtual filtering approach on a spectral flow cytometer (SFC-VF).
Original languageEnglish
Title of host publicationSpectral and Imaging Cytometry
Place of PublicationClifton, N-J
Pages23-40
Number of pages17
Volume2635
DOIs
Publication statusPublished - Apr 2023

Keywords

  • spectral flow cytometry
  • phytoplankton
  • ID7000
  • virtual filtering
  • spectral flow cytometer
  • cyanobacteria

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