Wet autoclave pretreatment for antigen retrieval in diagnostic immunohistochemistry

A. Bankfalvi, H. Navabi, B. Bier, W. Bocker, B. Jasani, K. W. Schmid

Research output: Contribution to journalArticlepeer-review

178 Citations (Scopus)

Abstract

Interest has recently been shown in adapting the microwave oven heating technique for antigen retrieval to routine diagnostic immunocytochemical practice. Although it has proved effective as a specialist method for individual antigen localization in many laboratories, it has certain drawbacks which have hampered its wider routine application. These include the need to monitor the sections during the microwave treatment to prevent damage or drying, the limited number of sections that can be accommodated in the microwave oven, and the inevitable alteration in nuclear morphology induced by the microwaves. In order to obviate these difficulties, we have modified the wet autoclave method of Shin et al. (Lab Invest 1991; 64: 693–702) as a routine technique for retrieval of a variety of cell surface, cytoplasmic, and nuclear antigens in formalin‐fixed and paraffin‐embedded tissue. The technique produces even enhancement of several refractory antigens in anatomically different sites and has the potential to handle reliably up to 200 sections at a time without significant damage to the section or to nuclear morphology.

Original languageEnglish
Pages (from-to)223-228
Number of pages6
JournalThe Journal of Pathology
Volume174
Issue number3
DOIs
Publication statusPublished - Nov 1994
Externally publishedYes

Keywords

  • Autoclave
  • antigen retrieval
  • immunocytochemistry
  • microwave
  • morphology
  • nuclear antigens

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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