FEUCHTES AUTOKLAVIEREN. DER EINFACHER WEG ZUR ANTIGENDEMASKIERUNG

Translated title of the contribution: Wet autoclave technique. The simpler method of antigen retrieval

A. Bankfalvi, K. Riehemann, D. Ofner, R. Checci, J. M. Morgan, J. Piffko, W. Bocker, B. Jasani, K. W. Schmid

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Wet autoclaving is a simple, reliable and time-effective method for antigen retrieval in routinely processed archival material. Both routine diagnostic (e.g., oestrogen and progesterone receptors, cytoskeletal proteins) and research antibodies (e.g. various p53 antibodies, mdm-2, bcl-2, MIB-1) are reported to demonstrate its application. Wet autoclaving may allow successful application of antibodies in paraffin-embedded tissues designed for use on frozen sections. The technique has the potential to reliably handle up to 200 sections at a time, without evidence of any significant damage to the sections or nuclear morphology.

Original languageGerman
Pages (from-to)345-349
Number of pages5
JournalPathologe
Volume15
Issue number6
DOIs
Publication statusPublished - 1994
Externally publishedYes

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Antigens
Antibodies
Cytoskeletal Proteins
Frozen Sections
Progesterone Receptors
Estrogen Receptors
Paraffin
Research

Keywords

  • antigen retrieval
  • immunohistochemistry
  • microwave pretreatment
  • wet autoclaving

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Bankfalvi, A., Riehemann, K., Ofner, D., Checci, R., Morgan, J. M., Piffko, J., ... Schmid, K. W. (1994). FEUCHTES AUTOKLAVIEREN. DER EINFACHER WEG ZUR ANTIGENDEMASKIERUNG. Pathologe, 15(6), 345-349. https://doi.org/10.1007/s002920050064

FEUCHTES AUTOKLAVIEREN. DER EINFACHER WEG ZUR ANTIGENDEMASKIERUNG. / Bankfalvi, A.; Riehemann, K.; Ofner, D.; Checci, R.; Morgan, J. M.; Piffko, J.; Bocker, W.; Jasani, B.; Schmid, K. W.

In: Pathologe, Vol. 15, No. 6, 1994, p. 345-349.

Research output: Contribution to journalArticle

Bankfalvi, A, Riehemann, K, Ofner, D, Checci, R, Morgan, JM, Piffko, J, Bocker, W, Jasani, B & Schmid, KW 1994, 'FEUCHTES AUTOKLAVIEREN. DER EINFACHER WEG ZUR ANTIGENDEMASKIERUNG', Pathologe, vol. 15, no. 6, pp. 345-349. https://doi.org/10.1007/s002920050064
Bankfalvi A, Riehemann K, Ofner D, Checci R, Morgan JM, Piffko J et al. FEUCHTES AUTOKLAVIEREN. DER EINFACHER WEG ZUR ANTIGENDEMASKIERUNG. Pathologe. 1994;15(6):345-349. https://doi.org/10.1007/s002920050064
Bankfalvi, A. ; Riehemann, K. ; Ofner, D. ; Checci, R. ; Morgan, J. M. ; Piffko, J. ; Bocker, W. ; Jasani, B. ; Schmid, K. W. / FEUCHTES AUTOKLAVIEREN. DER EINFACHER WEG ZUR ANTIGENDEMASKIERUNG. In: Pathologe. 1994 ; Vol. 15, No. 6. pp. 345-349.
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