FEUCHTES AUTOKLAVIEREN. DER EINFACHER WEG ZUR ANTIGENDEMASKIERUNG

A. Bankfalvi; K. Riehemann; D. Ofner; R. Checci; J. M. Morgan; J. Piffko; W. Bocker; B. Jasani; K. W. Schmid

doi:10.1007/s002920050064

FEUCHTES AUTOKLAVIEREN. DER EINFACHER WEG ZUR ANTIGENDEMASKIERUNG

Translated title of the contribution: Wet autoclave technique. The simpler method of antigen retrieval

A. Bankfalvi, K. Riehemann, D. Ofner, R. Checci, J. M. Morgan, J. Piffko, W. Bocker, B. Jasani, K. W. Schmid

School of Medicine

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

Wet autoclaving is a simple, reliable and time-effective method for antigen retrieval in routinely processed archival material. Both routine diagnostic (e.g., oestrogen and progesterone receptors, cytoskeletal proteins) and research antibodies (e.g. various p53 antibodies, mdm-2, bcl-2, MIB-1) are reported to demonstrate its application. Wet autoclaving may allow successful application of antibodies in paraffin-embedded tissues designed for use on frozen sections. The technique has the potential to reliably handle up to 200 sections at a time, without evidence of any significant damage to the sections or nuclear morphology.

Translated title of the contribution	Wet autoclave technique. The simpler method of antigen retrieval
Original language	German
Pages (from-to)	345-349
Number of pages	5
Journal	Pathologe
Volume	15
Issue number	6
DOIs	https://doi.org/10.1007/s002920050064
Publication status	Published - Dec 1 1994

Keywords

antigen retrieval
immunohistochemistry
microwave pretreatment
wet autoclaving

ASJC Scopus subject areas

Pathology and Forensic Medicine

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10.1007/s002920050064

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abstract = "Wet autoclaving is a simple, reliable and time-effective method for antigen retrieval in routinely processed archival material. Both routine diagnostic (e.g., oestrogen and progesterone receptors, cytoskeletal proteins) and research antibodies (e.g. various p53 antibodies, mdm-2, bcl-2, MIB-1) are reported to demonstrate its application. Wet autoclaving may allow successful application of antibodies in paraffin-embedded tissues designed for use on frozen sections. The technique has the potential to reliably handle up to 200 sections at a time, without evidence of any significant damage to the sections or nuclear morphology.",

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AU - Bankfalvi, A.

AU - Riehemann, K.

AU - Ofner, D.

AU - Checci, R.

AU - Morgan, J. M.

AU - Piffko, J.

AU - Bocker, W.

AU - Jasani, B.

AU - Schmid, K. W.

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