FEUCHTES AUTOKLAVIEREN. DER EINFACHER WEG ZUR ANTIGENDEMASKIERUNG

Translated title of the contribution: Wet autoclave technique. The simpler method of antigen retrieval

A. Bankfalvi, K. Riehemann, D. Ofner, R. Checci, J. M. Morgan, J. Piffko, W. Bocker, B. Jasani, K. W. Schmid

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)

Abstract

Wet autoclaving is a simple, reliable and time-effective method for antigen retrieval in routinely processed archival material. Both routine diagnostic (e.g., oestrogen and progesterone receptors, cytoskeletal proteins) and research antibodies (e.g. various p53 antibodies, mdm-2, bcl-2, MIB-1) are reported to demonstrate its application. Wet autoclaving may allow successful application of antibodies in paraffin-embedded tissues designed for use on frozen sections. The technique has the potential to reliably handle up to 200 sections at a time, without evidence of any significant damage to the sections or nuclear morphology.

Translated title of the contributionWet autoclave technique. The simpler method of antigen retrieval
Original languageGerman
Pages (from-to)345-349
Number of pages5
JournalPathologe
Volume15
Issue number6
DOIs
Publication statusPublished - Dec 1 1994

Keywords

  • antigen retrieval
  • immunohistochemistry
  • microwave pretreatment
  • wet autoclaving

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Fingerprint Dive into the research topics of 'Wet autoclave technique. The simpler method of antigen retrieval'. Together they form a unique fingerprint.

Cite this