Abstract
Wet autoclaving is a simple, reliable and time-effective method for antigen retrieval in routinely processed archival material. Both routine diagnostic (e.g., oestrogen and progesterone receptors, cytoskeletal proteins) and research antibodies (e.g. various p53 antibodies, mdm-2, bcl-2, MIB-1) are reported to demonstrate its application. Wet autoclaving may allow successful application of antibodies in paraffin-embedded tissues designed for use on frozen sections. The technique has the potential to reliably handle up to 200 sections at a time, without evidence of any significant damage to the sections or nuclear morphology.
| Original language | German |
|---|---|
| Pages (from-to) | 345-349 |
| Number of pages | 5 |
| Journal | Pathologe |
| Volume | 15 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 1994 |
| Externally published | Yes |
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Keywords
- antigen retrieval
- immunohistochemistry
- microwave pretreatment
- wet autoclaving
ASJC Scopus subject areas
- Pathology and Forensic Medicine
Cite this
FEUCHTES AUTOKLAVIEREN. DER EINFACHER WEG ZUR ANTIGENDEMASKIERUNG. / Bankfalvi, A.; Riehemann, K.; Ofner, D.; Checci, R.; Morgan, J. M.; Piffko, J.; Bocker, W.; Jasani, B.; Schmid, K. W.
In: Pathologe, Vol. 15, No. 6, 1994, p. 345-349.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - FEUCHTES AUTOKLAVIEREN. DER EINFACHER WEG ZUR ANTIGENDEMASKIERUNG
AU - Bankfalvi, A.
AU - Riehemann, K.
AU - Ofner, D.
AU - Checci, R.
AU - Morgan, J. M.
AU - Piffko, J.
AU - Bocker, W.
AU - Jasani, B.
AU - Schmid, K. W.
PY - 1994
Y1 - 1994
N2 - Wet autoclaving is a simple, reliable and time-effective method for antigen retrieval in routinely processed archival material. Both routine diagnostic (e.g., oestrogen and progesterone receptors, cytoskeletal proteins) and research antibodies (e.g. various p53 antibodies, mdm-2, bcl-2, MIB-1) are reported to demonstrate its application. Wet autoclaving may allow successful application of antibodies in paraffin-embedded tissues designed for use on frozen sections. The technique has the potential to reliably handle up to 200 sections at a time, without evidence of any significant damage to the sections or nuclear morphology.
AB - Wet autoclaving is a simple, reliable and time-effective method for antigen retrieval in routinely processed archival material. Both routine diagnostic (e.g., oestrogen and progesterone receptors, cytoskeletal proteins) and research antibodies (e.g. various p53 antibodies, mdm-2, bcl-2, MIB-1) are reported to demonstrate its application. Wet autoclaving may allow successful application of antibodies in paraffin-embedded tissues designed for use on frozen sections. The technique has the potential to reliably handle up to 200 sections at a time, without evidence of any significant damage to the sections or nuclear morphology.
KW - antigen retrieval
KW - immunohistochemistry
KW - microwave pretreatment
KW - wet autoclaving
UR - http://www.scopus.com/inward/record.url?scp=0028670469&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028670469&partnerID=8YFLogxK
U2 - 10.1007/s002920050064
DO - 10.1007/s002920050064
M3 - Article
VL - 15
SP - 345
EP - 349
JO - Der Pathologe
JF - Der Pathologe
SN - 0172-8113
IS - 6
ER -