Abstract
Wet autoclaving is a simple, reliable and time-effective method for antigen retrieval in routinely processed archival material. Both routine diagnostic (e.g., oestrogen and progesterone receptors, cytoskeletal proteins) and research antibodies (e.g. various p53 antibodies, mdm-2, bcl-2, MIB-1) are reported to demonstrate its application. Wet autoclaving may allow successful application of antibodies in paraffin-embedded tissues designed for use on frozen sections. The technique has the potential to reliably handle up to 200 sections at a time, without evidence of any significant damage to the sections or nuclear morphology.
Translated title of the contribution | Wet autoclave technique. The simpler method of antigen retrieval |
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Original language | German |
Pages (from-to) | 345-349 |
Number of pages | 5 |
Journal | Pathologe |
Volume | 15 |
Issue number | 6 |
DOIs | |
Publication status | Published - Dec 1 1994 |
Keywords
- antigen retrieval
- immunohistochemistry
- microwave pretreatment
- wet autoclaving
ASJC Scopus subject areas
- Pathology and Forensic Medicine