Whole-body UVB (TL-01) or UVA-1 irradiation does not alter the levels of immunomodulatory cytokines in the serum of human volunteers

P. M. McLoone, I. Man, S. Yule, A. Fluitman, H. van Loveren, M. Norval, N. K. Gibbs

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Background/Purpose: Ultraviolet (UV) exposure of mammalian skin induces local and systemic immunosuppression. In mice it has been proposed that systemic immunosuppression is mediated by an UV-induced cytokine cascade involving systemic interleukin (IL)-4 and IL-10 and a reduction in IL-12 activity. To investigate whether there was a parallel mechanism in humans we examined the effect of whole-body narrow-band ultraviolet B (UVB) (311-313 nm; TL-01) and ultraviolet A (UVA)-1 (340-400 nm) on serum cytokine levels. Methods/Results: In a first study, five male psoriatic subjects were whole-body irradiated with three sessions of a standard UVB (TL-01) phototherapy regimen previously shown to cause downregulation of natural killer cell activity and T helper 1 (Th1) and Th2 cytokine production by peripheral blood mononuclear cells. Enzyme-linked immunoabsorbent assay (ELISA) of sera taken before and after the third session showed no effect of phototherapy on IL-10 and tumour necrosis factor-α (TNF-α). In a second study, five healthy subjects received three whole-body exposures of UVB (TL-01) and five other healthy subjects received three exposures of UVA-1 on alternate days (total 22J/cm2). Blood samples were taken before the first irradiation and at 0, 4, 8, 12, 14, 24 and 48 h after the third irradiation. The sera were subsequently analysed for IL-10, IL-12, IL-8, IL-1β and TNF-α, by ELISA. The levels of IL-1β and TNF-α were below detection limits (

Original languageEnglish
Pages (from-to)76-80
Number of pages5
JournalPhotodermatology Photoimmunology and Photomedicine
Volume20
Issue number2
DOIs
Publication statusPublished - Apr 2004
Externally publishedYes

Fingerprint

Interleukin-10
Volunteers
Phototherapy
Tumor Necrosis Factor-alpha
Interleukin-12
Cytokines
Interleukin-1
Immunosuppression
Healthy Volunteers
Serum
Natural Killer T-Cells
Enzymes
Interleukin-8
Interleukin-4
Limit of Detection
Blood Cells
Down-Regulation
Skin

Keywords

  • Cytokines
  • UV-induced immunosuppression
  • Whole-body UV irradiation

ASJC Scopus subject areas

  • Dermatology
  • Immunology

Cite this

Whole-body UVB (TL-01) or UVA-1 irradiation does not alter the levels of immunomodulatory cytokines in the serum of human volunteers. / McLoone, P. M.; Man, I.; Yule, S.; Fluitman, A.; van Loveren, H.; Norval, M.; Gibbs, N. K.

In: Photodermatology Photoimmunology and Photomedicine, Vol. 20, No. 2, 04.2004, p. 76-80.

Research output: Contribution to journalArticle

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