Wild-type p53 induces apoptosis in Hep3B through up-regulation of bax expression

Ragai R. Mitry, Catherine E. Sarraf, Chuan Ging Wu, Massimo Pignatelli, Nagy A. Habib

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

We demonstrated that introduction and expression of wild-type p53 gene in the human hepatocellular carcinoma cell line, Hep3B, resulted in up- regulation of both p21(WAF/CIP1) and bax gene expression and apoptosis. This cell line contains integrated hepatitis B virus sequences and lacks the expression of both p53 and retinoblastoma tumor suppressor genes because of deletions. Our results suggest that whereas an increased level of bax expression mediates apoptosis, an increased level of p21 (WAF1/CIP1) expression does not induce arrest of cell growth, presumably because of the deletion of the retinoblastoma gene. This study also confirms reported observations that p53 is a tumor suppressor gene, which induces apoptosis in malignant cells that lack normal p53 activity because of mutation, deletion, or inactivation of the gene by the presence of oncogenic viral proteins.

Original languageEnglish
Pages (from-to)369-378
Number of pages10
JournalLaboratory Investigation
Volume77
Issue number4
Publication statusPublished - Oct 1997
Externally publishedYes

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Up-Regulation
Apoptosis
Tumor Suppressor Genes
Retinoblastoma Genes
Cell Line
Retinoblastoma
Sequence Deletion
p53 Genes
Gene Deletion
Gene Silencing
Viral Proteins
Hepatitis B virus
Hepatocellular Carcinoma
Gene Expression
Growth

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Mitry, R. R., Sarraf, C. E., Wu, C. G., Pignatelli, M., & Habib, N. A. (1997). Wild-type p53 induces apoptosis in Hep3B through up-regulation of bax expression. Laboratory Investigation, 77(4), 369-378.

Wild-type p53 induces apoptosis in Hep3B through up-regulation of bax expression. / Mitry, Ragai R.; Sarraf, Catherine E.; Wu, Chuan Ging; Pignatelli, Massimo; Habib, Nagy A.

In: Laboratory Investigation, Vol. 77, No. 4, 10.1997, p. 369-378.

Research output: Contribution to journalArticle

Mitry, RR, Sarraf, CE, Wu, CG, Pignatelli, M & Habib, NA 1997, 'Wild-type p53 induces apoptosis in Hep3B through up-regulation of bax expression', Laboratory Investigation, vol. 77, no. 4, pp. 369-378.
Mitry, Ragai R. ; Sarraf, Catherine E. ; Wu, Chuan Ging ; Pignatelli, Massimo ; Habib, Nagy A. / Wild-type p53 induces apoptosis in Hep3B through up-regulation of bax expression. In: Laboratory Investigation. 1997 ; Vol. 77, No. 4. pp. 369-378.
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