Abstract
The role of the transcription factor Yin Yang 1 (YY1) in development is largely unknown. Here we show that specific ablation of YY1 in mouse B cells caused a defect in somatic rearrangement in the immunoglobulin heavy-chain (IgH) locus and a block in the progenitor-B-to-precursor-B-cell transition, which was partially rescued by a prerearranged IgH transgene. Three-dimensional DNA fluorescence in situ hybridization analysis revealed an important function for YY1 in IgH locus contraction, a process indispensable for distal VH to DHJH recombination. We provide evidence that YY1 binds the intronic Eiμ enhancer within the IgH locus, consistent with a direct role for YY1 in VHDHJH recombination. These findings identified YY1 as a critical regulator of early B-cell development.
Original language | English |
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Pages (from-to) | 1179-1189 |
Number of pages | 11 |
Journal | Genes and Development |
Volume | 21 |
Issue number | 10 |
DOIs | |
Publication status | Published - May 15 2007 |
Keywords
- B-cell development
- Immunoglobulin gene
- V(D)J recombination
- YY1
ASJC Scopus subject areas
- Genetics
- Developmental Biology