Yin Yang 1 is a critical regulator of B-cell development

Huifei Liu; Marc Schmidt-Supprian; Yujiang Shi; Elias Hobeika; Natasha Barteneva; Hassan Jumaa; Roberta Pelanda; Michael Reth; Jane Skok; Klaus Rajewsky; Yang Shi

doi:10.1101/gad.1529307

Yin Yang 1 is a critical regulator of B-cell development

Huifei Liu, Marc Schmidt-Supprian, Yujiang Shi, Elias Hobeika, Natasha Barteneva, Hassan Jumaa, Roberta Pelanda, Michael Reth, Jane Skok, Klaus Rajewsky, Yang Shi

Department of Biology

Research output: Contribution to journal › Article › peer-review

188 Citations (Scopus)

Abstract

The role of the transcription factor Yin Yang 1 (YY1) in development is largely unknown. Here we show that specific ablation of YY1 in mouse B cells caused a defect in somatic rearrangement in the immunoglobulin heavy-chain (IgH) locus and a block in the progenitor-B-to-precursor-B-cell transition, which was partially rescued by a prerearranged IgH transgene. Three-dimensional DNA fluorescence in situ hybridization analysis revealed an important function for YY1 in IgH locus contraction, a process indispensable for distal V_H to D_HJ_H recombination. We provide evidence that YY1 binds the intronic Eiμ enhancer within the IgH locus, consistent with a direct role for YY1 in V_HD_HJ_H recombination. These findings identified YY1 as a critical regulator of early B-cell development.

Original language	English
Pages (from-to)	1179-1189
Number of pages	11
Journal	Genes and Development
Volume	21
Issue number	10
DOIs	https://doi.org/10.1101/gad.1529307
Publication status	Published - May 15 2007

Keywords

B-cell development
Immunoglobulin gene
V(D)J recombination
YY1

ASJC Scopus subject areas

Genetics
Developmental Biology

Access to Document

10.1101/gad.1529307

Fingerprint Dive into the research topics of 'Yin Yang 1 is a critical regulator of B-cell development'. Together they form a unique fingerprint.

Cite this

Yin Yang 1 is a critical regulator of B-cell development. / Liu, Huifei; Schmidt-Supprian, Marc; Shi, Yujiang; Hobeika, Elias; Barteneva, Natasha; Jumaa, Hassan; Pelanda, Roberta; Reth, Michael; Skok, Jane; Rajewsky, Klaus; Shi, Yang.

In: Genes and Development, Vol. 21, No. 10, 15.05.2007, p. 1179-1189.

Research output: Contribution to journal › Article › peer-review

@article{cf91a69285664525b4e2e924456ed5ea,

title = "Yin Yang 1 is a critical regulator of B-cell development",

abstract = "The role of the transcription factor Yin Yang 1 (YY1) in development is largely unknown. Here we show that specific ablation of YY1 in mouse B cells caused a defect in somatic rearrangement in the immunoglobulin heavy-chain (IgH) locus and a block in the progenitor-B-to-precursor-B-cell transition, which was partially rescued by a prerearranged IgH transgene. Three-dimensional DNA fluorescence in situ hybridization analysis revealed an important function for YY1 in IgH locus contraction, a process indispensable for distal VH to DHJH recombination. We provide evidence that YY1 binds the intronic Eiμ enhancer within the IgH locus, consistent with a direct role for YY1 in VHDHJH recombination. These findings identified YY1 as a critical regulator of early B-cell development.",

keywords = "B-cell development, Immunoglobulin gene, V(D)J recombination, YY1",

author = "Huifei Liu and Marc Schmidt-Supprian and Yujiang Shi and Elias Hobeika and Natasha Barteneva and Hassan Jumaa and Roberta Pelanda and Michael Reth and Jane Skok and Klaus Rajewsky and Yang Shi",

year = "2007",

month = may,

day = "15",

doi = "10.1101/gad.1529307",

language = "English",

volume = "21",

pages = "1179--1189",

journal = "Genes and Development",

issn = "0890-9369",

publisher = "Cold Spring Harbor Laboratory Press",

number = "10",

}

TY - JOUR

T1 - Yin Yang 1 is a critical regulator of B-cell development

AU - Liu, Huifei

AU - Schmidt-Supprian, Marc

AU - Shi, Yujiang

AU - Hobeika, Elias

AU - Barteneva, Natasha

AU - Jumaa, Hassan

AU - Pelanda, Roberta

AU - Reth, Michael

AU - Skok, Jane

AU - Rajewsky, Klaus

AU - Shi, Yang

PY - 2007/5/15

Y1 - 2007/5/15

N2 - The role of the transcription factor Yin Yang 1 (YY1) in development is largely unknown. Here we show that specific ablation of YY1 in mouse B cells caused a defect in somatic rearrangement in the immunoglobulin heavy-chain (IgH) locus and a block in the progenitor-B-to-precursor-B-cell transition, which was partially rescued by a prerearranged IgH transgene. Three-dimensional DNA fluorescence in situ hybridization analysis revealed an important function for YY1 in IgH locus contraction, a process indispensable for distal VH to DHJH recombination. We provide evidence that YY1 binds the intronic Eiμ enhancer within the IgH locus, consistent with a direct role for YY1 in VHDHJH recombination. These findings identified YY1 as a critical regulator of early B-cell development.

AB - The role of the transcription factor Yin Yang 1 (YY1) in development is largely unknown. Here we show that specific ablation of YY1 in mouse B cells caused a defect in somatic rearrangement in the immunoglobulin heavy-chain (IgH) locus and a block in the progenitor-B-to-precursor-B-cell transition, which was partially rescued by a prerearranged IgH transgene. Three-dimensional DNA fluorescence in situ hybridization analysis revealed an important function for YY1 in IgH locus contraction, a process indispensable for distal VH to DHJH recombination. We provide evidence that YY1 binds the intronic Eiμ enhancer within the IgH locus, consistent with a direct role for YY1 in VHDHJH recombination. These findings identified YY1 as a critical regulator of early B-cell development.

KW - B-cell development

KW - Immunoglobulin gene

KW - V(D)J recombination

KW - YY1

UR - http://www.scopus.com/inward/record.url?scp=34249035911&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34249035911&partnerID=8YFLogxK

U2 - 10.1101/gad.1529307

DO - 10.1101/gad.1529307

M3 - Article

C2 - 17504937

AN - SCOPUS:34249035911

VL - 21

SP - 1179

EP - 1189

JO - Genes and Development

JF - Genes and Development

SN - 0890-9369

IS - 10

ER -