Abstract
The role of the transcription factor Yin Yang 1 (YY1) in development is largely unknown. Here we show that specific ablation of YY1 in mouse B cells caused a defect in somatic rearrangement in the immunoglobulin heavy-chain (IgH) locus and a block in the progenitor-B-to-precursor-B-cell transition, which was partially rescued by a prerearranged IgH transgene. Three-dimensional DNA fluorescence in situ hybridization analysis revealed an important function for YY1 in IgH locus contraction, a process indispensable for distal VH to DHJH recombination. We provide evidence that YY1 binds the intronic Eiμ enhancer within the IgH locus, consistent with a direct role for YY1 in VHDHJH recombination. These findings identified YY1 as a critical regulator of early B-cell development.
| Original language | English |
|---|---|
| Pages (from-to) | 1179-1189 |
| Number of pages | 11 |
| Journal | Genes and Development |
| Volume | 21 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - May 15 2007 |
| Externally published | Yes |
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Keywords
- B-cell development
- Immunoglobulin gene
- V(D)J recombination
- YY1
ASJC Scopus subject areas
- Genetics
- Developmental Biology
Cite this
Yin Yang 1 is a critical regulator of B-cell development. / Liu, Huifei; Schmidt-Supprian, Marc; Shi, Yujiang; Hobeika, Elias; Barteneva, Natasha; Jumaa, Hassan; Pelanda, Roberta; Reth, Michael; Skok, Jane; Rajewsky, Klaus; Shi, Yang.
In: Genes and Development, Vol. 21, No. 10, 15.05.2007, p. 1179-1189.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Yin Yang 1 is a critical regulator of B-cell development
AU - Liu, Huifei
AU - Schmidt-Supprian, Marc
AU - Shi, Yujiang
AU - Hobeika, Elias
AU - Barteneva, Natasha
AU - Jumaa, Hassan
AU - Pelanda, Roberta
AU - Reth, Michael
AU - Skok, Jane
AU - Rajewsky, Klaus
AU - Shi, Yang
PY - 2007/5/15
Y1 - 2007/5/15
N2 - The role of the transcription factor Yin Yang 1 (YY1) in development is largely unknown. Here we show that specific ablation of YY1 in mouse B cells caused a defect in somatic rearrangement in the immunoglobulin heavy-chain (IgH) locus and a block in the progenitor-B-to-precursor-B-cell transition, which was partially rescued by a prerearranged IgH transgene. Three-dimensional DNA fluorescence in situ hybridization analysis revealed an important function for YY1 in IgH locus contraction, a process indispensable for distal VH to DHJH recombination. We provide evidence that YY1 binds the intronic Eiμ enhancer within the IgH locus, consistent with a direct role for YY1 in VHDHJH recombination. These findings identified YY1 as a critical regulator of early B-cell development.
AB - The role of the transcription factor Yin Yang 1 (YY1) in development is largely unknown. Here we show that specific ablation of YY1 in mouse B cells caused a defect in somatic rearrangement in the immunoglobulin heavy-chain (IgH) locus and a block in the progenitor-B-to-precursor-B-cell transition, which was partially rescued by a prerearranged IgH transgene. Three-dimensional DNA fluorescence in situ hybridization analysis revealed an important function for YY1 in IgH locus contraction, a process indispensable for distal VH to DHJH recombination. We provide evidence that YY1 binds the intronic Eiμ enhancer within the IgH locus, consistent with a direct role for YY1 in VHDHJH recombination. These findings identified YY1 as a critical regulator of early B-cell development.
KW - B-cell development
KW - Immunoglobulin gene
KW - V(D)J recombination
KW - YY1
UR - http://www.scopus.com/inward/record.url?scp=34249035911&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34249035911&partnerID=8YFLogxK
U2 - 10.1101/gad.1529307
DO - 10.1101/gad.1529307
M3 - Article
VL - 21
SP - 1179
EP - 1189
JO - Genes and Development
JF - Genes and Development
SN - 0890-9369
IS - 10
ER -