Yin Yang 1 is a critical regulator of B-cell development

Huifei Liu; Marc Schmidt-Supprian; Yujiang Shi; Elias Hobeika; Natasha Barteneva; Hassan Jumaa; Roberta Pelanda; Michael Reth; Jane Skok; Klaus Rajewsky; Yang Shi

doi:10.1101/gad.1529307

Yin Yang 1 is a critical regulator of B-cell development

Huifei Liu, Marc Schmidt-Supprian, Yujiang Shi, Elias Hobeika, Natasha Barteneva, Hassan Jumaa, Roberta Pelanda, Michael Reth, Jane Skok, Klaus Rajewsky, Yang Shi

Department of Biology

Research output: Contribution to journal › Article

184 Citations (Scopus)

Abstract

The role of the transcription factor Yin Yang 1 (YY1) in development is largely unknown. Here we show that specific ablation of YY1 in mouse B cells caused a defect in somatic rearrangement in the immunoglobulin heavy-chain (IgH) locus and a block in the progenitor-B-to-precursor-B-cell transition, which was partially rescued by a prerearranged IgH transgene. Three-dimensional DNA fluorescence in situ hybridization analysis revealed an important function for YY1 in IgH locus contraction, a process indispensable for distal V_H to D_HJ_H recombination. We provide evidence that YY1 binds the intronic Eiμ enhancer within the IgH locus, consistent with a direct role for YY1 in V_HD_HJ_H recombination. These findings identified YY1 as a critical regulator of early B-cell development.

Original language	English
Pages (from-to)	1179-1189
Number of pages	11
Journal	Genes and Development
Volume	21
Issue number	10
DOIs	https://doi.org/10.1101/gad.1529307
Publication status	Published - May 15 2007

Keywords

B-cell development
Immunoglobulin gene
V(D)J recombination
YY1

ASJC Scopus subject areas

Genetics
Developmental Biology

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Liu, H., Schmidt-Supprian, M., Shi, Y., Hobeika, E., Barteneva, N., Jumaa, H., Pelanda, R., Reth, M., Skok, J., Rajewsky, K., & Shi, Y. (2007). Yin Yang 1 is a critical regulator of B-cell development. Genes and Development, 21(10), 1179-1189. https://doi.org/10.1101/gad.1529307

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abstract = "The role of the transcription factor Yin Yang 1 (YY1) in development is largely unknown. Here we show that specific ablation of YY1 in mouse B cells caused a defect in somatic rearrangement in the immunoglobulin heavy-chain (IgH) locus and a block in the progenitor-B-to-precursor-B-cell transition, which was partially rescued by a prerearranged IgH transgene. Three-dimensional DNA fluorescence in situ hybridization analysis revealed an important function for YY1 in IgH locus contraction, a process indispensable for distal VH to DHJH recombination. We provide evidence that YY1 binds the intronic Eiμ enhancer within the IgH locus, consistent with a direct role for YY1 in VHDHJH recombination. These findings identified YY1 as a critical regulator of early B-cell development.",

keywords = "B-cell development, Immunoglobulin gene, V(D)J recombination, YY1",

author = "Huifei Liu and Marc Schmidt-Supprian and Yujiang Shi and Elias Hobeika and Natasha Barteneva and Hassan Jumaa and Roberta Pelanda and Michael Reth and Jane Skok and Klaus Rajewsky and Yang Shi",

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AU - Liu, Huifei

AU - Schmidt-Supprian, Marc

AU - Shi, Yujiang

AU - Hobeika, Elias

AU - Barteneva, Natasha

AU - Jumaa, Hassan

AU - Pelanda, Roberta

AU - Reth, Michael

AU - Skok, Jane

AU - Rajewsky, Klaus

AU - Shi, Yang

PY - 2007/5/15

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N2 - The role of the transcription factor Yin Yang 1 (YY1) in development is largely unknown. Here we show that specific ablation of YY1 in mouse B cells caused a defect in somatic rearrangement in the immunoglobulin heavy-chain (IgH) locus and a block in the progenitor-B-to-precursor-B-cell transition, which was partially rescued by a prerearranged IgH transgene. Three-dimensional DNA fluorescence in situ hybridization analysis revealed an important function for YY1 in IgH locus contraction, a process indispensable for distal VH to DHJH recombination. We provide evidence that YY1 binds the intronic Eiμ enhancer within the IgH locus, consistent with a direct role for YY1 in VHDHJH recombination. These findings identified YY1 as a critical regulator of early B-cell development.

AB - The role of the transcription factor Yin Yang 1 (YY1) in development is largely unknown. Here we show that specific ablation of YY1 in mouse B cells caused a defect in somatic rearrangement in the immunoglobulin heavy-chain (IgH) locus and a block in the progenitor-B-to-precursor-B-cell transition, which was partially rescued by a prerearranged IgH transgene. Three-dimensional DNA fluorescence in situ hybridization analysis revealed an important function for YY1 in IgH locus contraction, a process indispensable for distal VH to DHJH recombination. We provide evidence that YY1 binds the intronic Eiμ enhancer within the IgH locus, consistent with a direct role for YY1 in VHDHJH recombination. These findings identified YY1 as a critical regulator of early B-cell development.

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KW - V(D)J recombination

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