Yin Yang 1 is a critical regulator of B-cell development

Huifei Liu, Marc Schmidt-Supprian, Yujiang Shi, Elias Hobeika, Natasha Barteneva, Hassan Jumaa, Roberta Pelanda, Michael Reth, Jane Skok, Klaus Rajewsky, Yang Shi

Research output: Contribution to journalArticle

177 Citations (Scopus)

Abstract

The role of the transcription factor Yin Yang 1 (YY1) in development is largely unknown. Here we show that specific ablation of YY1 in mouse B cells caused a defect in somatic rearrangement in the immunoglobulin heavy-chain (IgH) locus and a block in the progenitor-B-to-precursor-B-cell transition, which was partially rescued by a prerearranged IgH transgene. Three-dimensional DNA fluorescence in situ hybridization analysis revealed an important function for YY1 in IgH locus contraction, a process indispensable for distal VH to DHJH recombination. We provide evidence that YY1 binds the intronic Eiμ enhancer within the IgH locus, consistent with a direct role for YY1 in VHDHJH recombination. These findings identified YY1 as a critical regulator of early B-cell development.

Original languageEnglish
Pages (from-to)1179-1189
Number of pages11
JournalGenes and Development
Volume21
Issue number10
DOIs
Publication statusPublished - May 15 2007
Externally publishedYes

Fingerprint

Yin-Yang
Immunoglobulin Heavy Chains
B-Lymphocytes
Genetic Recombination
B-Lymphoid Precursor Cells
Fluorescence In Situ Hybridization
Transgenes
Transcription Factors
DNA

Keywords

  • B-cell development
  • Immunoglobulin gene
  • V(D)J recombination
  • YY1

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

Liu, H., Schmidt-Supprian, M., Shi, Y., Hobeika, E., Barteneva, N., Jumaa, H., ... Shi, Y. (2007). Yin Yang 1 is a critical regulator of B-cell development. Genes and Development, 21(10), 1179-1189. https://doi.org/10.1101/gad.1529307

Yin Yang 1 is a critical regulator of B-cell development. / Liu, Huifei; Schmidt-Supprian, Marc; Shi, Yujiang; Hobeika, Elias; Barteneva, Natasha; Jumaa, Hassan; Pelanda, Roberta; Reth, Michael; Skok, Jane; Rajewsky, Klaus; Shi, Yang.

In: Genes and Development, Vol. 21, No. 10, 15.05.2007, p. 1179-1189.

Research output: Contribution to journalArticle

Liu, H, Schmidt-Supprian, M, Shi, Y, Hobeika, E, Barteneva, N, Jumaa, H, Pelanda, R, Reth, M, Skok, J, Rajewsky, K & Shi, Y 2007, 'Yin Yang 1 is a critical regulator of B-cell development', Genes and Development, vol. 21, no. 10, pp. 1179-1189. https://doi.org/10.1101/gad.1529307
Liu H, Schmidt-Supprian M, Shi Y, Hobeika E, Barteneva N, Jumaa H et al. Yin Yang 1 is a critical regulator of B-cell development. Genes and Development. 2007 May 15;21(10):1179-1189. https://doi.org/10.1101/gad.1529307
Liu, Huifei ; Schmidt-Supprian, Marc ; Shi, Yujiang ; Hobeika, Elias ; Barteneva, Natasha ; Jumaa, Hassan ; Pelanda, Roberta ; Reth, Michael ; Skok, Jane ; Rajewsky, Klaus ; Shi, Yang. / Yin Yang 1 is a critical regulator of B-cell development. In: Genes and Development. 2007 ; Vol. 21, No. 10. pp. 1179-1189.
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