TY - GEN
T1 - Brillouin light scattering spectroscopy for tissue engineering application
AU - Akilbekova, Dana
AU - Yakupov, Talgat
AU - Ogay, Vyacheslav
AU - Umbayev, Bauyrzhan
AU - Yakovlev, Vladislav V.
AU - Utegulov, Zhandos N.
N1 - Funding Information:
Acknowledgements: This work was supported by Nazarbayev University grant SST0142015 and MES RK state-targeted program BR05236454.
PY - 2018/1/1
Y1 - 2018/1/1
N2 - Biomechanical properties of mammalian bones, such as strength, toughness and plasticity, are essential for understanding how microscopic scale mechanical features can link to macroscale bones' strength and fracture resistance. We employ Brillouin light scattering (BLS) micro-spectroscopy for local assessment of elastic properties of bones under compression and the efficacy of the tissue engineering approach based on heparin-conjugated fibrin (HCF) hydrogels, bone morphogenic proteins (BMPs) and osteogenic stem cells in the regeneration of the bone tissues. BLS is noninvasive and label-free imaging modality for probing mechanical properties of hard tissues that can give information on structure-function properties of normal and pathological tissues. Results showed that HCF gels containing combination of all factors had the best effect with complete defect regeneration at week 9 and that the bones with fully consolidated fractures have higher values of elastic moduli compared to the bones with defects.
AB - Biomechanical properties of mammalian bones, such as strength, toughness and plasticity, are essential for understanding how microscopic scale mechanical features can link to macroscale bones' strength and fracture resistance. We employ Brillouin light scattering (BLS) micro-spectroscopy for local assessment of elastic properties of bones under compression and the efficacy of the tissue engineering approach based on heparin-conjugated fibrin (HCF) hydrogels, bone morphogenic proteins (BMPs) and osteogenic stem cells in the regeneration of the bone tissues. BLS is noninvasive and label-free imaging modality for probing mechanical properties of hard tissues that can give information on structure-function properties of normal and pathological tissues. Results showed that HCF gels containing combination of all factors had the best effect with complete defect regeneration at week 9 and that the bones with fully consolidated fractures have higher values of elastic moduli compared to the bones with defects.
KW - biomechanical properties
KW - bone morphogenic proteins
KW - bones
KW - Brillouin light scattering
KW - critical sized defect
KW - heparin-conjugated fibrin gel
KW - mesenchymal stem cells
UR - https://www.scopus.com/pages/publications/85045146781
UR - https://www.scopus.com/pages/publications/85045146781#tab=citedBy
U2 - 10.1117/12.2289923
DO - 10.1117/12.2289923
M3 - Conference contribution
AN - SCOPUS:85045146781
VL - 10496
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Optical Elastography and Tissue Biomechanics V
A2 - Sampson, David D.
A2 - Larin, Kirill V.
PB - SPIE
T2 - Optical Elastography and Tissue Biomechanics V 2018
Y2 - 27 January 2018 through 28 January 2018
ER -