TY - JOUR
T1 - Cell sorting in cancer research-Diminishing degree of cell heterogeneity
AU - Barteneva, Natasha S.
AU - Ketman, Kenneth
AU - Fasler-Kan, Elizaveta
AU - Potashnikova, Daria
AU - Vorobjev, Ivan A.
N1 - Funding Information:
We would like to apologize to the authors of the many relevant works that were not cited. Funding was provided in part by Harvard's Pilot Grant and the Immune Disease Institute to N.S.B. and RFBR grants 11-01517a and 11-01749a to I.A.V. D.P. was supported by ICRETT fellowship from International Union Against Cancer. This work was supported in part by M.V. Lomonosov Moscow State University Program of Development . We are very grateful to Dr. Isabel Beerman for sharing her stem cell data with us. We also want to acknowledge Olga Khudoleeva for help with preparation of manuscript figures. We also thank Aleksandra Gorelova (Harvard University) for help with the editing of the manuscript.
PY - 2013/8
Y1 - 2013/8
N2 - Increasing evidence of intratumor heterogeneity and its augmentation due to selective pressure of microenvironment and recent achievements in cancer therapeutics lead to the need to investigate and track the tumor subclonal structure. Cell sorting of heterogeneous subpopulations of tumor and tumor-associated cells has been a long established strategy in cancer research. Advancement in lasers, computer technology and optics has led to a new generation of flow cytometers and cell sorters capable of high-speed processing of single cell suspensions. Over the last several years cell sorting was used in combination with molecular biological methods, imaging and proteomics to characterize primary and metastatic cancer cell populations, minimal residual disease and single tumor cells. It was the principal method for identification and characterization of cancer stem cells. Analysis of single cancer cells may improve early detection of tumors, monitoring of circulating tumor cells, evaluation of intratumor heterogeneity and chemotherapeutic treatments. The aim of this review is to provide an overview of major cell sorting applications and approaches with new prospective developments such as microfluidics and microchip technologies.
AB - Increasing evidence of intratumor heterogeneity and its augmentation due to selective pressure of microenvironment and recent achievements in cancer therapeutics lead to the need to investigate and track the tumor subclonal structure. Cell sorting of heterogeneous subpopulations of tumor and tumor-associated cells has been a long established strategy in cancer research. Advancement in lasers, computer technology and optics has led to a new generation of flow cytometers and cell sorters capable of high-speed processing of single cell suspensions. Over the last several years cell sorting was used in combination with molecular biological methods, imaging and proteomics to characterize primary and metastatic cancer cell populations, minimal residual disease and single tumor cells. It was the principal method for identification and characterization of cancer stem cells. Analysis of single cancer cells may improve early detection of tumors, monitoring of circulating tumor cells, evaluation of intratumor heterogeneity and chemotherapeutic treatments. The aim of this review is to provide an overview of major cell sorting applications and approaches with new prospective developments such as microfluidics and microchip technologies.
KW - Cancer
KW - Cancer heterogeneity
KW - Cell heterogeneity
KW - Flow cytometry
KW - High-speed cell sorting
KW - Microarray
UR - https://www.scopus.com/pages/publications/84877828458
UR - https://www.scopus.com/pages/publications/84877828458#tab=citedBy
U2 - 10.1016/j.bbcan.2013.02.004
DO - 10.1016/j.bbcan.2013.02.004
M3 - Review article
C2 - 23481260
AN - SCOPUS:84877828458
SN - 0304-419X
VL - 1836
SP - 105
EP - 122
JO - Biochimica et Biophysica Acta - Reviews on Cancer
JF - Biochimica et Biophysica Acta - Reviews on Cancer
IS - 1
ER -
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