TY - JOUR
T1 - Molecular and Cellular Mechanism of Action of Chrysotile Asbestos in MRC5 Cell Line
AU - Kussainova, Assiya
AU - Bulgakova, Olga
AU - Aripova, Akmaral
AU - Ibragimova, Milana
AU - Pulliero, Alessandra
AU - Begimbetova, Dinara
AU - Bersimbaev, Rakhmetkazhi
AU - Izzotti, Alberto
N1 - Publisher Copyright:
© 2023 by the authors.
PY - 2023/11
Y1 - 2023/11
N2 - Asbestos is a known carcinogen; however, the influence of chrysotile asbestos on the development of tumor-related diseases remains a subject of intense debate within the scientific community. To analyze the effect of asbestos, we conducted a study using the MRC5 cell line. We were able to demonstrate that chrysotile asbestos stimulated the production of reactive oxygen species (ROS), leading to cell death and DNA damage in the MRC5 cell line, using various techniques such as ROS measurement, comet assay, MTT assay, and qPCR. In addition, we found that chrysotile asbestos treatment significantly increased extracellular mitochondrial DNA levels in the culture medium and induced significant changes in the expression profile of several miRNAs, which was the first of its kind. Thus, our research highlights the importance of studying the effects of chrysotile asbestos on human health and reveals multiple adverse effects of chrysotile asbestos.
AB - Asbestos is a known carcinogen; however, the influence of chrysotile asbestos on the development of tumor-related diseases remains a subject of intense debate within the scientific community. To analyze the effect of asbestos, we conducted a study using the MRC5 cell line. We were able to demonstrate that chrysotile asbestos stimulated the production of reactive oxygen species (ROS), leading to cell death and DNA damage in the MRC5 cell line, using various techniques such as ROS measurement, comet assay, MTT assay, and qPCR. In addition, we found that chrysotile asbestos treatment significantly increased extracellular mitochondrial DNA levels in the culture medium and induced significant changes in the expression profile of several miRNAs, which was the first of its kind. Thus, our research highlights the importance of studying the effects of chrysotile asbestos on human health and reveals multiple adverse effects of chrysotile asbestos.
KW - cf mtDNA
KW - chrysotile asbestos
KW - lung cancer
KW - microRNA
KW - reactive oxygen species
UR - https://www.scopus.com/pages/publications/85178282238
UR - https://www.scopus.com/inward/citedby.url?scp=85178282238&partnerID=8YFLogxK
U2 - 10.3390/jpm13111599
DO - 10.3390/jpm13111599
M3 - Article
AN - SCOPUS:85178282238
SN - 2075-4426
VL - 13
JO - Journal of Personalized Medicine
JF - Journal of Personalized Medicine
IS - 11
M1 - 1599
ER -
