TY - CHAP
T1 - Spectral and imaging flow cytometry in phytoplankton research
AU - Dashkova, Veronika
AU - Clapper, Jeff
AU - Vorobyev, Ivan
AU - Barteneva, Natalie
PY - 2018/1/1
Y1 - 2018/1/1
N2 - Spectral and imaging flow cytometry are emerging technologies that allow quantifying spectral, fluorescent, and/or morphological parameters of heterogeneous cellular populations. The protocol describes a detailed step-by-step analysis of microalgae using these techniques and examples from our laboratory (Aphanizomenon sp., Cryptomonas pyrenoidifera, and Chlorella sp.). Moreover, the chapter will be helpful to scientists who want to perform spectral flow cytometry and apply principal component analysis.
AB - Spectral and imaging flow cytometry are emerging technologies that allow quantifying spectral, fluorescent, and/or morphological parameters of heterogeneous cellular populations. The protocol describes a detailed step-by-step analysis of microalgae using these techniques and examples from our laboratory (Aphanizomenon sp., Cryptomonas pyrenoidifera, and Chlorella sp.). Moreover, the chapter will be helpful to scientists who want to perform spectral flow cytometry and apply principal component analysis.
KW - Imaging flow cytometry
KW - Microalgae
KW - Population heterogeneity
KW - Programmed cell death
KW - Spectral flow cytometry
UR - https://www.scopus.com/pages/publications/85042564958
UR - https://www.scopus.com/pages/publications/85042564958#tab=citedBy
U2 - 10.1007/978-1-4939-7680-5_5
DO - 10.1007/978-1-4939-7680-5_5
M3 - Chapter
AN - SCOPUS:85042564958
T3 - Methods in Molecular Biology
SP - 83
EP - 95
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -